Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
Monoclonal Antibody: SDOW17
Monoclonal antibody SDOW17 recognizes a highly conserved, conformationally dependant epitope on the nucleocapsid protein of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). This antibody reacts with 99% of North American and European strains of PRRSV. It does not react with the PrimePac PRRSV vaccine strain. The product is supplied as mouse ascites fluid (SDOW17-A), mouse ascites conjugated with fluorescein isothiocyanate (SDOW17-F), and mouse ascites conjugated with biotin (SDOW17-B).
Non-neutralizing
Uses
SDOW17-A
Product SDOW17-A is recommended for use in immunohistochemistry, indirect fluorescent antibody, and enzyme linked-immunosorbent assay applications. Individual laboratories and investigators are advised to determine the optimal working dilution for their protocol.
SDOW17-F
This monoclonal antibody-fluorescein conjugate is recommended for use in immunofluorescence tests at a dilution not to exceed 1:100 in phosphate buffered saline.
SDOW17-B
This monoclonal antibody-biotin conjugate is recommended for use in enzyme-linked immunosorbent assays for antigen detection. Working dilutions should not exceed 1:500.
Cost
Product: SDOW17-Ascites
Lot Number: 1009282
Prices:
SDOW17-A (1ml aliquots)........$385.00
SDOW17-F (1ml aliquots)........ $385.00
SDOW17-B (100 µl aliquots).......$70.00
SDOW17-B (1 ml aliquots)........$610.00
Isotype
IgG1
Uses and Recommended Dilutions
This antibody is provided sterile-filtered. Shelf life at -20°C is a minimum of 5 years. Avoid repeated freeze-thaw cycles. Upon receipt, antibody may be thawed, dispensed in small aliquots and frozen. Antibody should be diluted in phosphate buffered saline (PBS) or PBS+1% bovine serum albumin (BSA).
Citations:
1. Weller, TM; D Horter; JM Neiger; EA Nelson. 1999. World Association of Veterinary Laboratory Diagnosticians. IX International Symposium, June 2-5, College Station, TX. Pg.135.
2. Schoper, JM; EA Nelson. 1997. In Proceedings of the 78th Annual Meeting of the Conference of Research Workers in Animal Diseases. November 10-11, Madison, WI. Abstract p 110.
3. Nelson, EA; J Christoper-Hennings; T Drew, et al. 1993. Journal of Clinical Microbiology. December. Vol. 31, No. 12. p 3184-3189.