Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
Monoclonal Antibody: SR30
Monoclonal antibody SR30 recognizes a highly conserved, conformationally dependant epitope on the nucleocapsid protein of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). This antibody reacts with virtually all North American and European strains of PRRSV. The product is supplied as mouse ascites fluid (SR30-A), mouse ascites conjugated with fluorescein isothiocyanate (SR30-F), and mouse ascites conjugated with biotin (SR30-B).
Non-neutralizing
Uses
Parental Myeloma: NS-1
SR30-A
Product SR30-A is recommended for use in immunohistochemistry, indirect fluorescent antibody, and enzyme linked-immunosorbent assay applications. Individual laboratories and investigators are advised to determine the optimal working dilution for their protocol.
SR30-F
This monoclonal antibody-fluorescein conjugate is recommended for use in immunofluorescence tests at a dilution not to exceed 1:100 in phosphate buffered saline.
SR30-B
This monoclonal antibody-biotin conjugate is recommended for use in enzyme-linked immunosorbent assays for antigen detection. Working dilutions should not exceed 1:500.
Cost
Prices:
SR30-A (1ml aliquots).........$385.00
SR30-F (1ml aliquots)..........$385.00
SR30-B (100 µl aliquots).......$70.00
SR30-B (1 ml aliquots).........$610.00
Isotype
IgG1
Uses and Recommended Dilutions
This antibody is provided sterile-filtered. Shelf life at -20°C is a minimum of 5 years. Avoid repeated freeze-thaw cycles. Upon receipt, antibody may be thawed, dispensed in small aliquots and frozen. Antibody should be diluted in phosphate buffered saline (PBS) or PBS+1% bovine serum albumin (BSA).
1. Nelson, EA, JK Nelson, J Christopher-Hennings, P Steen, KJ Yoon, R Hesse, C Chase, & D Benfield. 1995. Reactivity of PRRSV field isolates with a panel of monoclonal antibodies. 76th Annual Meeting of the Conference of Research Workers in Animal Diseases, Nov 13-14, Chicago, IL. Abstract p 89.
2. Nelson, EA, JK Nelson, J Christopher-Hennings, KJ Yoon, R Magar and DA Benfield. 1996. Reactivity of North American PRRSV isolates with a monoclonal antibody panel. Proceedings of the 14th International Pig Veterinary Society Congress, July 7-10, Bologna, Italy, p 88.